Expert Antibody Validation for Flow Cytometry
High-quality flow cytometry data depends entirely on the quality of your antibodies. An unvalidated antibody can lead to false positives, incorrect population gating, and irreproducible results. SauveBio provides specialized antibody validation services for flow cytometry, ensuring your reagents perform with the specificity and consistency required for clear, accurate, and publishable data. Move forward with confidence in every channel.
Our Flow Cytometry Validation Services
We offer a multi-parameter approach to rigorously assess your antibody’s performance for flow cytometry, providing you with a complete characterization profile. Our antibody validation services can be used for:
- Unconjugated Primary Antibodies
- Directly Conjugated (DC) Antibodies
- Secondary Antibodies
Core Validation Assays:
- Optimal Titer Determination: We perform comprehensive titration analysis on relevant cell types to identify the ideal antibody concentration that maximizes the signal-to-noise ratio, preventing background noise and conserving your valuable reagent.
- Specificity Confirmation: We test whether your antibody binds to the correct target using a variety of robust methods.
- Positive & Negative Cell Lines: Staining of cell lines known to express or lack the target of interest provides clear evidence of specificity.
- Knockout (KO) Validation: As the gold standard, we use CRISPR-generated knockout cell lines to definitively demonstrate that the signal is absent without the target protein.
- Isotype Controls: We establish baseline fluorescence and assess non-specific Fc receptor binding using appropriate isotype controls.
- Performance on Primary Cells: We can test and verify antibody performance on specific primary cell populations (CD4+ T cells, B cells, monocytes, etc.) to ensure it works in your experimental system.
- Intracellular Staining Validation: We optimize and validate antibodies for intracellular targets, including verification of the best fixation and permeabilization protocol.
Why SauveBio for Flow Cytometry?
Partner with a team that understands the nuances of multi-color flow cytometric analysis.
- Deep Expertise: Our scientists specialize in immunology, cell biology, and the technical complexities of flow cytometry panel design, instrumentation, and data analysis.
- Publication-Ready Reports: You receive a detailed report including all plots, gating strategies, Median/Mean Fluorescence Intensity (MFI) values, instrument settings, and a clear summary of the results, in addition to the raw data files.
- Customized for Your Research: We consult with you to design a validation strategy that matches your specific cell types, species, and experimental goals, from surface to intracellular targets.
- Advanced Instrumentation: We leverage state-of-the-art, multi-parameter flow cytometers to generate the high-resolution data your research demands.
Our Simple Process
- Consult: Discuss your antibody, target population, and experimental context with our flow cytometry specialists.
- Submit: Send us your antibody and any specific cell line or protocol information.
- Validate: We execute the agreed-upon validation assays with the highest standards of scientific rigor.
- Report: You receive a comprehensive data package, empowering you to move forward with total confidence.
Ready to eliminate antibody uncertainty from your flow cytometry experiments?
Visit www.sauvebio.com or email us at info@sauvebio.com to start your project today!
