At SauveBio, we are dedicated to pushing the boundaries of scientific research through innovative technologies. One such groundbreaking technique is Fluorescence Activated Mitochondria Sorting (FAMS), a nanoscale method of flow cytometry that has the power to revolutionize our understanding of mitochondrial biology. Let’s explore the advanced features of FAMS and its contributions to the field, referencing MacDonald et al. 2019 and Sheehan et al. 2023. Our CEO & CSO, Hannah Sheehan, participated in the validation and publication of this method during her time at Northeastern University pursuing her Ph.D. in Biology.
What is FAMS?
Fluorescence Activated Mitochondria Sorting (FAMS) is a cutting-edge technique that leverages the principles of flow cytometry to sort and analyze mitochondria based on their unique fluorescent properties. This method allows researchers to assess mitochondria as individual entities, providing unprecedented insights into mitochondrial heterogeneity and function.
Key Features and Advantages
- High Sensitivity and Resolution: FAMS enables the detection and quantification of mitochondrial parameters at the single-organelle level, offering a higher level of sensitivity and resolution than traditional methods. This is crucial for identifying subtle variations in mitochondrial DNA content, membrane potential, and protein expression.
- Multiparametric Analysis: The technique allows for the simultaneous measurement of multiple parameters, such as mitochondrial membrane potential, size, and protein expression. This comprehensive view of mitochondrial function is essential for understanding various disease states and aging.
- Speed and Efficiency: FAMS can analyze thousands of mitochondria per second, making it a fast and efficient method for studying mitochondrial dynamics. This high throughput capability is particularly beneficial for large-scale studies and clinical applications.
- Quantitative Data: The technique provides quantitative data, allowing for precise measurements and comparisons between different samples or experimental conditions. This quantitative approach enhances the reliability and reproducibility of research findings.
- Staining of Live Cells for Analysis: FAMS involves staining cells while they are alive, preserving the physiological state of the mitochondria and providing more relevant biological insights. This feature is particularly important for studying mitochondrial function in real-time.
Significant Contributions to the Field
MacDonald et al. 2019 [1]: This study introduced the concept of nanoscale fluorescence-activated mitochondrial sorting (FAMS). This method allows for the sorting of mitochondrial subpopulations with minimal impact on viability while achieving high throughput, yield, and purity. This innovative approach has paved the way for more detailed and accurate analysis of mitochondrial function.
Sheehan et al. 2023 [2]: Building on the advancements of MacDonald et al., Sheehan et al. 2023 further refined the FAMS technique, enabling the separation of individual mitochondria belonging to subpopulations of interest using fluorescent dyes and antibody labeling. This detailed protocol highlighted the significant variability in mitochondrial functional, physical, and chemical attributes, which are relevant to various disease states and aging. By examining mitochondria individually, researchers can gain deeper insights into metabolic disorders, neurodegenerative diseases, and cancer.
Why FAMS Matters to Your Research
FAMS stands apart by enabling the isolation and analysis of distinct mitochondrial subpopulations that whole-cell analysis might overlook. This capability is vital for researchers seeking to understand how specific mitochondrial subpopulations influence disease progression and treatment outcomes. By providing a novel tool to detect differences between samples, FAMS enhances the ability of researchers to conduct high-resolution studies on mitochondrial function and pathology.
Conclusion
Fluorescence Activated Mitochondria Sorting (FAMS) is more than just a technique; it’s a transformative tool that empowers researchers to unlock the mysteries of mitochondrial biology. At SauveBio, we are committed to harnessing the power of FAMS to drive scientific breakthroughs and support our clients’ research endeavors. By leveraging this innovative technology, we aim to accelerate the discovery of new therapeutic targets and improve our understanding of complex diseases.
Ready to elevate your research? Contact SauveBio today to learn how our advanced sorting methods can accelerate your scientific discoveries.
References
[1] J.A. MacDonald, A.M. Bothun, S.N. Annis, H. Sheehan, S. Ray, Y. Gao, A.R. Ivanov, K. Khrapko, J.L. Tilly, and D.C. Woods, A nanoscale, multi-parametric flow cytometry-based platform to study mitochondrial heterogeneity and mitochondrial DNA dynamics. Commun Biol 2 (2019) 258.
[2] H.C. Sheehan, J.L. Tilly, and D.C. Woods, Assaying Mitochondrial Function by Multiparametric Flow Cytometry. Methods Mol Biol 2644 (2023) 65-80.