SauveBio is pleased to announce the publication of a cutting-edge study co-authored by our Co‑Founder, CEO & CSO, Dr. Hannah C. Sheehan. The paper, titled “Flow Cytometric Quantification of Mitochondrial Properties: A High‑Throughput Approach for Single Organelle Analysis”, was recently released in the International Journal of Molecular Sciences.
Building on their previously validated method, fluorescence‑activated mitochondria sorting (FAMS), the team utilizes FAMS to allow for detailed, individual-organelle analysis.
Key achievements include:
- Quantitative enumeration of mitochondrial subpopulations based on size, membrane potential, and surface markers
- Tracking the translocation of proteins linked to mitophagy, using PINK1 and Parkin probes
- Detection and characterization of free-floating and extracellular vesicle (EV)–encapsulated mitochondria
- Measurement of mitochondrial DNA (mtDNA) content alongside functional parameters
This multi-parametric platform offers a powerful new framework for exploring mitochondrial heterogeneity, autophagy, and intercellular organelle signaling – areas of considerable importance to disease research and therapeutic innovation.
At SauveBio, we’re excited to see this methodology amplify the resolution of cellular analysis and enrich research across biology, immunology, and therapeutic development. We’re proud to contribute to the advancement of organelle-scale science—and grateful to our co-authors (Piasecki, Tilly, and Woods) and Northeastern University collaborators for bringing this innovation to publication.
📘 Read the full article: International Journal of Molecular Sciences, June 2025, Vol. 26, Issue 12, Article 5481
Piasecki, A.J.; Sheehan, H.C.; Tilly, J.L.; Woods, D.C. Flow Cytometric Quantification of Mitochondrial Properties: A High-Throughput Approach for Single Organelle Analysis. Int. J. Mol. Sci. 2025, 26, 5481. https://doi.org/10.3390/ijms26125481
Blog post by Reagan Cleversey (https://www.linkedin.com/in/reagan-cleversey/)