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At SauveBio, we are dedicated to pushing the boundaries of scientific research through innovative technologies. One such groundbreaking technique is Fluorescence Activated Mitochondria Sorting (FAMS), a nanoscale method of flow cytometry that has the power to revolutionize our understanding of mitochondrial biology. Let’s explore the advanced features of FAMS and its contributions to the field, referencing MacDonald et al. 2019 and Sheehan et al. 2023. Our CEO & CSO, Hannah Sheehan, participated in the validation and publication of this method during her time at Northeastern University pursuing her Ph.D. in Biology.

What is FAMS?

Fluorescence Activated Mitochondria Sorting (FAMS) is a cutting-edge technique that leverages the principles of flow cytometry to sort and analyze mitochondria based on their unique fluorescent properties. This method allows researchers to assess mitochondria as individual entities, providing unprecedented insights into mitochondrial heterogeneity and function.

Key Features and Advantages

1. High Sensitivity and Resolution: FAMS enables the detection and quantification of mitochondrial parameters at the single-organelle level, offering a higher level of sensitivity and resolution than traditional methods. This is crucial for identifying subtle variations in mitochondrial DNA content, membrane potential, and protein expression.
2. Multiparametric Analysis: The technique allows for the simultaneous measurement of multiple parameters, such as mitochondrial membrane potential, size, and protein expression. This comprehensive view of mitochondrial function is essential for understanding various disease states and aging.
3. Speed and Efficiency: FAMS can analyze thousands of mitochondria per second, making it a fast and efficient method for studying mitochondrial dynamics. This high throughput capability is particularly beneficial for large-scale studies and clinical applications.
4. Quantitative Data: The technique provides quantitative data, allowing for precise measurements and comparisons between different samples or experimental conditions. This quantitative approach enhances the reliability and reproducibility of research findings.
5. Staining of Live Cells for Analysis: FAMS involves staining cells while they are alive, preserving the physiological state of the mitochondria and providing more relevant biological insights. This feature is particularly important for studying mitochondrial function in real-time.

Significant Contributions to the Field

MacDonald et al. 2019 [1]: This study introduced the concept of nanoscale fluorescence-activated mitochondrial sorting (FAMS). This method allows for the sorting of mitochondrial subpopulations with minimal impact on viability while achieving high throughput, yield, and purity. This innovative approach has paved the way for more detailed and accurate analysis of mitochondrial function.

Sheehan et al. 2023 [2]: Building on the advancements of MacDonald et al., Sheehan et al. 2023 further refined the FAMS technique, enabling the separation of individual mitochondria belonging to subpopulations of interest using fluorescent dyes and antibody labeling. This detailed protocol highlighted the significant variability in mitochondrial functional, physical, and chemical attributes, which are relevant to various disease states and aging. By examining mitochondria individually, researchers can gain deeper insights into metabolic disorders, neurodegenerative diseases, and cancer.

Why FAMS Matters to Your Research

FAMS stands apart by enabling the isolation and analysis of distinct mitochondrial subpopulations that whole-cell analysis might overlook. This capability is vital for researchers seeking to understand how specific mitochondrial subpopulations influence disease progression and treatment outcomes. By providing a novel tool to detect differences between samples, FAMS enhances the ability of researchers to conduct high-resolution studies on mitochondrial function and pathology.

Conclusion

Fluorescence Activated Mitochondria Sorting (FAMS) is more than just a technique; it’s a transformative tool that empowers researchers to unlock the mysteries of mitochondrial biology. At SauveBio, we are committed to harnessing the power of FAMS to drive scientific breakthroughs and support our clients’ research endeavors. By leveraging this innovative technology, we aim to accelerate the discovery of new therapeutic targets and improve our understanding of complex diseases.

Ready to elevate your research? Contact SauveBio today to learn how our advanced sorting methods can accelerate your scientific discoveries. 

References 

[1] J.A. MacDonald, A.M. Bothun, S.N. Annis, H. Sheehan, S. Ray, Y. Gao, A.R. Ivanov, K. Khrapko, J.L. Tilly, and D.C. Woods, A nanoscale, multi-parametric flow cytometry-based platform to study mitochondrial heterogeneity and mitochondrial DNA dynamics. Commun Biol 2 (2019) 258.

[2] H.C. Sheehan, J.L. Tilly, and D.C. Woods, Assaying Mitochondrial Function by Multiparametric Flow Cytometry. Methods Mol Biol 2644 (2023) 65-80.

At SauveBio, we are committed to providing our clients with the most advanced and reliable tools for their research needs. One of the standout instruments in our arsenal is the Thermo Attune NxT Flow Cytometer. This cutting-edge device is designed to deliver exceptional performance and precision, making it an invaluable asset for any research lab. Let’s explore the advanced features that set the Thermo Attune NxT apart from the competition.

Acoustic Focusing Technology

The Thermo Attune NxT Flow Cytometer utilizes revolutionary acoustic focusing technology, which employs ultrasonic waves to precisely align cells within the flow cell [1]. This innovative approach ensures that cells are tightly focused at the point of laser interrogation, resulting in less signal variation and better data quality. This technology allows for high-speed sample acquisition and exceptional sensitivity, making it ideal for detecting rare events and analyzing low-concentration samples [1].

High-Speed Sample Acquisition

One of the key advantages of the Thermo Attune NxT is its ability to rapidly acquire high-quality data. With sample throughput rates of up to 12.5 µL/min or 1,000 µL/min, and acquisition speeds of up to 35,000 events per second, the Attune NxT significantly reduces the time required to process samples [1]. This means researchers can achieve faster results without compromising on data quality.

Flexible Optical Design

The Thermo Attune NxT offers a flexible optical design that can be configured with up to four lasers, including yellow and green, for up to 14 color panels [1]. This flexibility allows researchers to customize the instrument to meet their specific experimental needs, enabling the simultaneous detection of multiple parameters and enhancing the overall efficiency of their workflows.

Clog-Resistant Fluidics

The Attune NxT’s clog-resistant fluidics system ensures maximal uptime and minimal maintenance [1]. This feature is particularly beneficial for labs handling a high volume of samples, as it reduces the risk of interruptions and ensures consistent performance throughout the day.

Exceptional Sensitivity

The Thermo Attune NxT is designed to deliver exceptional sensitivity, allowing researchers to detect even the most subtle differences in fluorescence intensity between cell populations [1]. This high level of sensitivity is crucial for applications such as cell cycle analysis, where precise detection of fluorescence intensity is essential for accurate results.

Why Choose SauveBio?

At SauveBio, we combine the power of the Thermo Attune NxT with our technical expertise to offer unparalleled flow cytometry services. Our personalized and collaborative approach ensures direct communication between researchers and our scientists, providing customized experimental design, data analysis, and expert guidance. Founded by industry experts Hannah Sheehan, Ph.D. and Jeff Sauve, SauveBio blends scientific expertise with enhanced support services to deliver tailored solutions for biotech and pharma clients.

Conclusion

The Thermo Attune NxT Flow Cytometer is more than just an instrument; it’s a game-changer in the field of flow cytometry. With its advanced features and exceptional performance, it empowers researchers to achieve new levels of precision and efficiency in their work. At SauveBio, we are dedicated to harnessing the power of this innovative technology to drive scientific breakthroughs and support our clients’ research endeavors.

Ready to elevate your research? Contact SauveBio today to learn how our advanced flow cytometry services can accelerate your scientific discoveries.

References

[1] Thermo Attune.